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KMID : 0900920000240020143
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Korean journal of Animal Reproduction
2000 Volume.24 No. 2 p.143 ~ p.154
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Aberrant Microtubule Assembly and Chromatin Configuration of Homan Oocytes Which Failed to Complete Fertilization Following In Vitro Fertilization and Intracytoplasmic Sperm Injection
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Chung H.-M.
Lim J.-M.
Park C.
Ko J.-J.
Cha K.-Y.
Kim J.-M.
Chung K.-S.
Kim N.-H.
Kim J.-W.
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Abstract
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Most eggs initiated the fertilization processes but arrested at specific stages. The stages included failure of the oocyte to exit from the meiotic metaphase-II with or without sperm penetration, failure of appropriate sperm aster formation, inability to form proper male and female pronuclei, failure of suitable pronuclear apposition, and failure to form proper number of either male or female pronuclei. Various images of defective microtubule organization and chromatin configuration during IVF and ICSI procedures were observed. We discussed the data with previous research results during normal fertilization in humans and other mammals. In conclusion, various aberrant patterns in microtubule assembly and chromatin configuration, which were assessed in the present study, could be used as criteria to improve assisted reproductive technology in clinics. However, further cellular and molecular characterization is needed to clarify these aberrant patterns of cytoskeletal assembly.
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KEYWORD
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Microtubules, In vitro fertilization, Intracytoplasmic sperm injection, Human eggs
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